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FANCF Polyclonal antibody

FANCF Polyclonal Antibody for IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, ELISA

Conjugate

Unconjugated

Cat no : 18060-1-AP

Print datasheet

Synonyms

FAF, FANCF, Fanconi anemia group F protein, Protein FACF

验证数据展示

  • IP experiment of HeLa using 18060-1-AP

    IP experiment of HeLa using 18060-1-AP

    IP result of anti-FANCF (IP:18060-1-AP, 4ug; Detection:18060-1-AP 1:400) with HeLa cells lysate 1600 ug.

经过测试的应用

Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18060-1-AP targets FANCF in IP, ELISA applications and shows reactivity with human samples.

Tested Applications IP, ELISA Application Description
Tested Reactivity human
Immunogen FANCF fusion protein Ag12649 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fanconi anemia, complementation group F
Synonyms FAF, FANCF, Fanconi anemia group F protein, Protein FACF
Calculated Molecular Weight 374 aa, 42 kDa
Observed Molecular Weight36 kDa
GenBank Accession NumberBC093867
Gene Symbol FANCF
Gene ID (NCBI) 2188
RRIDAB_3669289
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPI8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Fanconi anemia complement group F (FANCF) is located on chromosome 11p15, a region enriched in cancer-associated genes. FANCF is known to be involved in DNA repair, and the overexpression of FANCF protein leads to cell proliferation and ultimately to cancer. The epigenetic silencing of FANCF has been reported in several different tumors such as ovaries, bladder, cervical, leukemic, testicular, lung, and oral tumors (PMID: 21915857, 32915143).

实验方案

Product Specific Protocols
IP protocol for FANCF antibody 18060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols