FANCI Polyclonal antibody

FANCI Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 20789-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA and More (1)

Protein FACI, Fanconi anemia group I protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20789-1-AP targets FANCI in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, CoIP
经测试反应性 human
文献引用反应性human
免疫原 FANCI fusion protein Ag14386 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Fanconi anemia, complementation group I
别名 Protein FACI, Fanconi anemia group I protein
计算分子量 1328 aa, 149 kDa
观测分子量 149 kDa
GenBank蛋白编号BC004277
基因名称 FANCI
Gene ID (NCBI) 55215
RRIDAB_10694829
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NVI1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FANCI antibody 20789-1-APDownload protocol
IHC protocol for FANCI antibody 20789-1-APDownload protocol
IP protocol for FANCI antibody 20789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cancer

Aberrant R-loop-mediated immune evasion, cellular communication, and metabolic reprogramming affect cancer progression: a single-cell analysis

Authors - Shichao Zhang
humanWB

Sci Adv

Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair

Authors - Dinh Duc Nguyen
humanWB,CoIP

Cell Death Dis

Inactivation of ribosomal protein S27-like impairs DNA interstrand cross-link repair by destabilization of FANCD2 and FANCI.

Authors - Siyuan Sun
human

Adv Sci (Weinh)

Phosphorylation of USP29 by CDK1 Governs TWIST1 Stability and Oncogenic Functions

Authors - Tangming Guan
WB

Mol Carcinog

KNTC1 knockdown inhibits the proliferation and migration of osteosarcoma cells by MCM2

Authors - Lei Zhong
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