验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| CoIP | See 1 publications below |
产品信息
20789-1-AP targets FANCI in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB, CoIP |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | FANCI fusion protein Ag14386 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Fanconi anemia, complementation group I |
| Synonyms | Protein FACI, Fanconi anemia group I protein |
| Calculated Molecular Weight | 1328 aa, 149 kDa |
| Observed Molecular Weight | 149 kDa |
| GenBank Accession Number | BC004277 |
| Gene Symbol | FANCI |
| Gene ID (NCBI) | 55215 |
| RRID | AB_10694829 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NVI1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FANCI antibody 20789-1-AP | Download protocol |
| IHC protocol for FANCI antibody 20789-1-AP | Download protocol |
| IP protocol for FANCI antibody 20789-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer Aberrant R-loop-mediated immune evasion, cellular communication, and metabolic reprogramming affect cancer progression: a single-cell analysis | ||
Sci Adv Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair | ||
Cell Death Dis Inactivation of ribosomal protein S27-like impairs DNA interstrand cross-link repair by destabilization of FANCD2 and FANCI. | ||
Adv Sci (Weinh) Phosphorylation of USP29 by CDK1 Governs TWIST1 Stability and Oncogenic Functions | ||
Mol Carcinog KNTC1 knockdown inhibits the proliferation and migration of osteosarcoma cells by MCM2 |