验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A431 cells, HEK-293 cells, Jurkat cells, K-562 cells, LNCaP cells, A549 cells, Raji cells, Daudi cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
67304-1-Ig targets FANCI in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | FANCI fusion protein Ag15153 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | Fanconi anemia, complementation group I |
| Synonyms | Protein FACI, Fanconi anemia group I protein |
| Calculated Molecular Weight | 1328 aa, 149 kDa |
| Observed Molecular Weight | 149 kDa, 142 kDa |
| GenBank Accession Number | BC004277 |
| Gene Symbol | FANCI |
| Gene ID (NCBI) | 55215 |
| RRID | AB_2882566 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NVI1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FANCI antibody 67304-1-Ig | Download protocol |
| IHC protocol for FANCI antibody 67304-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) HPV Enhances HNSCC Chemosensitization by Inhibiting SERPINB3 Expression to Disrupt the Fanconi Anemia Pathway | ||
Front Immunol FANCI serve as a prognostic biomarker correlated with immune infiltrates in skin cutaneous melanoma | ||
Adv Sci (Weinh) The Compromised Fanconi Anemia Pathway in Prelamin A-Expressing Cells Contributes to Replication Stress-Induced Genomic Instability |
