验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, A431 cells, HEK-293 cells, Jurkat cells, K-562 cells, LNCaP cells, A549 cells, Raji cells, Daudi cells |
Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 3 publications below |
IHC | See 1 publications below |
产品信息
67304-1-Ig targets FANCI in WB, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | FANCI fusion protein Ag15153 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | Fanconi anemia, complementation group I |
别名 | Protein FACI, Fanconi anemia group I protein |
计算分子量 | 1328 aa, 149 kDa |
观测分子量 | 149 kDa, 142 kDa |
GenBank蛋白编号 | BC004277 |
基因名称 | FANCI |
Gene ID (NCBI) | 55215 |
RRID | AB_2882566 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q9NVI1 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for FANCI antibody 67304-1-Ig | Download protocol |
IHC protocol for FANCI antibody 67304-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) HPV Enhances HNSCC Chemosensitization by Inhibiting SERPINB3 Expression to Disrupt the Fanconi Anemia Pathway | ||
Front Immunol FANCI serve as a prognostic biomarker correlated with immune infiltrates in skin cutaneous melanoma | ||
Adv Sci (Weinh) The Compromised Fanconi Anemia Pathway in Prelamin A-Expressing Cells Contributes to Replication Stress-Induced Genomic Instability |