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FANCL Polyclonal antibody

FANCL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14454-1-AP

Print datasheet

Synonyms

FAAP43, FANCL, Fanconi anemia group L protein, PHF9, POG

验证数据展示

  • WB analysis of HEK-293 using 14454-1-AP

    WB analysis of HEK-293 using 14454-1-AP

    WB result of FANCL antibody (14454-1-AP; 1:200; incubated at room temperature for 1.5 hours) with sh-Control and sh-FANCL transfected HEK-293 cells.

  • WB analysis of A549 using 14454-1-AP

    WB analysis of A549 using 14454-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 14454-1-AP (FANCL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 14454-1-AP

    IHC staining of human kidney using 14454-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14454-1-AP (FANCL Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 14454-1-AP

    IHC staining of human kidney using 14454-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14454-1-AP (FANCL Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HEK-cells, Hela cells, HepG2 cells
Positive IHC detected inhuman kidney tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14454-1-AP targets FANCL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen FANCL fusion protein Ag6031 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fanconi anemia, complementation group L
Synonyms FAAP43, FANCL, Fanconi anemia group L protein, PHF9, POG
Calculated Molecular Weight 43 kDa
Observed Molecular Weight28 kDa
GenBank Accession NumberBC054517
Gene Symbol FANCL
Gene ID (NCBI) 55120
RRIDAB_10644333
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NW38
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fanconi anemia (FA) is a hereditary bone marrow failure syndrome associated with developmental defects and cancer predisposition. The FA nuclear core complex consists of FANCA, B, C, E, F, G, L, and M. FANCL is a RING-type E3 ubiquitin ligase that monoubiquitinates FANCD2 and FANCI, a key functional role facilitated by other members of the nuclear core complex (PMID: 23783032). FANCL plays an important role in the repair of cisplatin-induced DNA damage (PMID: 26385482) and participates directly in support of stem cell function (PMID: 23783032). FANCL can be detected as 40-43 kDa (full-length) and ~28 kDa.

实验方案

Product Specific Protocols
WB protocol for FANCL antibody 14454-1-APDownload protocol
IHC protocol for FANCL antibody 14454-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols