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验证数据展示

  • WB analysis of HeLa using 66639-1-Ig (same clone as 66639-1-PBS)

    WB analysis of HeLa using 66639-1-Ig (same clone as 66639-1-PBS)

    HeLa cells and HEK-293 cells were subjected to SDS PAGE followed by western blot with 66639-1-Ig (FANCL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66639-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66639-1-Ig (same clone as 66639-1-PBS)

    WB analysis of HEK-293 using 66639-1-Ig (same clone as 66639-1-PBS)

    WB result of FANCL antibody (66639-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FANCL transfected HEK-293 cells. This data was developed using the same antibody clone with 66639-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66639-1-Ig (same clone as 66639-1-PBS)

    IF Staining of HeLa using 66639-1-Ig (same clone as 66639-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCL antibody (66639-1-Ig, Clone: 6E2B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66639-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

FANCL Monoclonal antibody, PBS Only

FANCL Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66639-1-PBS

产品说明书

CloneNo. 6E2B6

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, IF/ICC, Indirect ELISA

别名

FAAP43, FANCL, Fanconi anemia group L protein, PHF9, POG

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66639-1-PBS targets FANCL in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human
免疫原 FANCL fusion protein Ag6192 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 Fanconi anemia, complementation group L
别名 FAAP43, FANCL, Fanconi anemia group L protein, PHF9, POG
计算分子量 43 kDa
观测分子量 41-43 kDa
GenBank蛋白编号BC054517
基因名称 FANCL
Gene ID (NCBI) 55120
RRIDAB_2881998
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NW38
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fanconi anemia (FA) is a hereditary bone marrow failure syndrome associated with developmental defects and cancer predisposition. The FA nuclear core complex consists of FANCA, B, C, E, F, G, L, and M. FANCL is a RING-type E3 ubiquitin ligase that monoubiquitinates FANCD2 and FANCI, a key functional role facilitated by other members of the nuclear core complex (PMID: 23783032). FANCL plays an important role in the repair of cisplatin-induced DNA damage (PMID: 26385482) and participates directly in support of stem cell function (PMID: 23783032). FANCL can be detected as 40-43 kDa (full-length) and ~28 kDa.