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验证数据展示

  • IHC staining of mouse testis using 82897-2-RR

    IHC staining of mouse testis using 82897-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82897-2-RR (FANCM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 82897-2-RR

    IHC staining of mouse testis using 82897-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82897-2-RR (FANCM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of PC-3 using 82897-2-RR

    FC experiment of PC-3 using 82897-2-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug FANCM Recombinant antibody (82897-2-RR, Clone:230233G5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IP experiment of HepG2 using 82897-2-RR

    IP experiment of HepG2 using 82897-2-RR

    IP result of anti-FANCM (IP:82897-2-RR, 4ug; Detection:82897-2-RR 1:500) with HepG2 cells lysate 400 ug.

  • IF Staining of HeLa using 82897-2-RR

    IF Staining of HeLa using 82897-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCM antibody (82897-2-RR, Clone: 230233G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

FANCM Recombinant antibody

FANCM Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 82897-2-RR

产品说明书

CloneNo. 230233G5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), IP, ELISA

别名

Fanconi anemia-associated polypeptide of 250 kDa, Fanconi anemia group M protein, FAAP250, EC:3.6.4.13, ATP-dependent RNA helicase FANCM

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IP detected inHepG2 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82897-2-RR targets FANCM in IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原 FANCM fusion protein Ag34447 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fanconi anemia, complementation group M
别名 Fanconi anemia-associated polypeptide of 250 kDa, Fanconi anemia group M protein, FAAP250, EC:3.6.4.13, ATP-dependent RNA helicase FANCM
GenBank蛋白编号BC140776
基因名称 FANCM
Gene ID (NCBI) 57697
RRIDAB_3670625
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IYD8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for FANCM antibody 82897-2-RRDownload protocol
IF protocol for FANCM antibody 82897-2-RRDownload protocol
IP protocol for FANCM antibody 82897-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols