验证数据展示
经过测试的应用
| Positive WB detected in | U-87 MG cells, U2OS cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 2 publications below |
产品信息
11779-1-AP targets FAPA in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | FAPA fusion protein Ag2439 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | fibroblast activation protein, alpha |
| Synonyms | FAP, EC:3.4.21.26, EC:3.4.21.-, EC:3.4.14.5, Dipeptidyl peptidase FAP |
| Calculated Molecular Weight | 760 aa, 88 kDa |
| Observed Molecular Weight | 87-97 kDa |
| GenBank Accession Number | BC026250 |
| Gene Symbol | FAP |
| Gene ID (NCBI) | 2191 |
| RRID | AB_3669133 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12884 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fibroblast activation protein-alpha (FAP-alpha) is a cell surface glycoprotein serine protease, which can participate in extracellular matrix degradation and is involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth (PMID: 14751930; 16223769). FAP-alpha is a single transmembrane protein that locates the cytoplasm and plasma membrane. Immunohistochemical studies have shown that FAP-alpha is transiently expressed in certain normal fetal mesenchymal tissues but that normal adult tissues as well as malignant epithelial, neural, and hematopoietic cells are generally FAP-alpha-negative. However, most of the common types of epithelial cancers contain abundant FAP-alpha-reactive stromal fibroblasts (PMID: 7911242).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FAPA antibody 11779-1-AP | Download protocol |
| IHC protocol for FAPA antibody 11779-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Theranostics LINC00982-encoded protein PRDM16-DT regulates CHEK2 splicing to suppress colorectal cancer metastasis and chemoresistance | ||
Mol Cancer Extracellular vesicles of carcinoma-associated fibroblasts creates a pre-metastatic niche in the lung through activating fibroblasts. | ||
Mol Biol Rep Cancer-associated fibroblast-derived exosome Leptin promotes malignant biological lineage in pancreatic ductal adenocarcinoma by regulating ABL2 via miR-224-3p |