验证数据展示
经过测试的应用
Positive WB detected in | U-87 MG cells, U2OS cells |
Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IF | See 2 publications below |
产品信息
11779-1-AP targets FAP in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human |
文献引用反应性 | human, mouse |
免疫原 | FAP fusion protein Ag2439 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | fibroblast activation protein, alpha |
别名 | FAPA, 170 kDa melanoma membrane-bound gelatinase, Antiplasmin-cleaving enzyme FAP, soluble form, Dipeptidyl peptidase FAP, EC:3.4.14.5 |
计算分子量 | 760 aa, 88 kDa |
观测分子量 | 87-97 kDa |
GenBank蛋白编号 | BC026250 |
基因名称 | FAP |
Gene ID (NCBI) | 2191 |
RRID | AB_3669133 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q12884 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fibroblast activation protein-alpha (FAP-alpha) is a cell surface glycoprotein serine protease, which can participate in extracellular matrix degradation and is involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth (PMID: 14751930; 16223769). FAP-alpha is a single transmembrane protein that locates the cytoplasm and plasma membrane. Immunohistochemical studies have shown that FAP-alpha is transiently expressed in certain normal fetal mesenchymal tissues but that normal adult tissues as well as malignant epithelial, neural, and hematopoietic cells are generally FAP-alpha-negative. However, most of the common types of epithelial cancers contain abundant FAP-alpha-reactive stromal fibroblasts (PMID: 7911242).
实验方案
Product Specific Protocols | |
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WB protocol for FAP antibody 11779-1-AP | Download protocol |
IHC protocol for FAP antibody 11779-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Theranostics LINC00982-encoded protein PRDM16-DT regulates CHEK2 splicing to suppress colorectal cancer metastasis and chemoresistance | ||
Mol Cancer Extracellular vesicles of carcinoma-associated fibroblasts creates a pre-metastatic niche in the lung through activating fibroblasts. | ||
Mol Biol Rep Cancer-associated fibroblast-derived exosome Leptin promotes malignant biological lineage in pancreatic ductal adenocarcinoma by regulating ABL2 via miR-224-3p |