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验证数据展示

  • WB analysis using 27717-1-AP

    WB analysis using 27717-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27717-1-AP (FAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 27717-1-AP

    IHC staining of human colon using 27717-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27717-1-AP (FAR2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FAR2 Polyclonal antibody

FAR2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27717-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SDR10E2, MLSTD1, HEL-S-81

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNeuro-2a cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27717-1-AP targets FAR2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FAR2 fusion protein Ag26819 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fatty acyl CoA reductase 2
别名 SDR10E2, MLSTD1, HEL-S-81
计算分子量 515 aa, 59 kDa
观测分子量59 kDa
GenBank蛋白编号BC022267
基因名称 FAR2
Gene ID (NCBI) 55711
RRIDAB_3669618
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96K12
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fatty acyl-CoA reductase 2 (FAR2), also known as MLSTD1, belongs to the fatty acyl-CoA reductase family. FAR2 catalyzes the reduction of saturated but not unsaturated C16 or C18 fatty acyl-CoA to fatty alcohols (PMID: 24009241). FAR2 may play a role in the production of ether lipids/plasmalogens and wax monoesters which synthesis requires fatty alcohols as substrates. FAR2 has 2 isoforms with the molecular mass of 49 kDa and 59 kDa (Refer to Uniprot).

实验方案

Product Specific Protocols
WB protocol for FAR2 antibody 27717-1-APDownload protocol
IHC protocol for FAR2 antibody 27717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols