• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 24014-1-AP

    WB analysis using 24014-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24014-1-AP (FARP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 24014-1-AP

    IP experiment of HeLa using 24014-1-AP

    IP result of anti-FARP1 (IP:24014-1-AP, 4ug; Detection:24014-1-AP 1:1000) with HeLa cells lysate 1200 ug.

FARP1 Polyclonal antibody

FARP1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 24014-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

Pleckstrin homology domain-containing family C member 2, PH domain-containing family C member 2, FERM, RhoGEF and pleckstrin domain-containing protein 1, FERM, ARHGEF and pleckstrin domain-containing protein 1, Chondrocyte-derived ezrin-like protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, HEK-293 cells, HeLa cells
Positive IP detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24014-1-AP targets FARP1 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原 FARP1 fusion protein Ag21225 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived)
别名 Pleckstrin homology domain-containing family C member 2, PH domain-containing family C member 2, FERM, RhoGEF and pleckstrin domain-containing protein 1, FERM, ARHGEF and pleckstrin domain-containing protein 1, Chondrocyte-derived ezrin-like protein
计算分子量 1076 aa, 122 kDa
观测分子量122 kDa
GenBank蛋白编号BC071592
基因名称 FARP1
Gene ID (NCBI) 10160
RRIDAB_3085720
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9Y4F1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FARP1 is a protein containing a FERM (4.2, exrin, radixin, moesin) domain, a Dbl homology domain, and two pleckstrin homology domains. These domains are found in guanine nucleotide exchange factors and proteins that link the cytoskeleton to the cell membrane. The encoded protein functions in neurons to promote dendritic growth.

实验方案

Product Specific Protocols
WB protocol for FARP1 antibody 24014-1-APDownload protocol
IP protocol for FARP1 antibody 24014-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols