验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
16436-1-AP targets FARS2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | FARS2 fusion protein Ag9750 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phenylalanyl-tRNA synthetase 2, mitochondrial |
| 别名 | FARS1, FARS2, HSPC320, Phenylalanine tRNA ligase, PheRS |
| 计算分子量 | 451 aa, 52 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC021112 |
| 基因名称 | FARS2 |
| Gene ID (NCBI) | 10667 |
| RRID | AB_2102499 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95363 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FARS2, a nuclear gene located on chromosome 6 (6p25.1), encodes the mitochondrial phenylalanyl-tRNA synthetase (mtPheRS), which transfers phenylalanine (Phe) to its cognate tRNA in mitochondria (PMID: 32774346).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FARS2 antibody 16436-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Genet Metab Clinical and molecular characterization of novel FARS2 variants causing neonatal mitochondrial disease | ||
Biochim Biophys Acta Mutation of the human mitochondrial phenylalanine-tRNA synthetase causes infantile-onset epilepsy and cytochrome c oxidase deficiency. | ||
Eur J Hum Genet Novel FARS2 variants in patients with early onset encephalopathy with or without epilepsy associated with long survival. | ||
Nat Commun Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ. |