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FARSA Monoclonal antibody

FARSA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D3E2

Cat no : 67945-1-Ig

Print datasheet

Synonyms

FARSLA, FARSL, FARS, EC:6.1.1.20, CML33

验证数据展示

  • WB analysis using 67945-1-Ig

    WB analysis using 67945-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67945-1-Ig (FARSA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human breast cancer using 67945-1-Ig

    IHC staining of human breast cancer using 67945-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67945-1-Ig (FARSA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FARSA antibody included
  • IHC staining of human breast cancer using 67945-1-Ig

    IHC staining of human breast cancer using 67945-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67945-1-Ig (FARSA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FARSA antibody included
  • IF Staining of A431 using 67945-1-Ig

    IF Staining of A431 using 67945-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FARSA antibody (67945-1-Ig, Clone: 2D3E2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

经过测试的应用

Positive WB detected inLNCaP cells, HSC-T6 cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy FARSA Ready-To-Use IHC Kit. FARSA primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67945-1-Ig targets FARSA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen FARSA fusion protein Ag12647 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name phenylalanyl-tRNA synthetase, alpha subunit
Synonyms FARSLA, FARSL, FARS, EC:6.1.1.20, CML33
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC006495
Gene Symbol FARSA
Gene ID (NCBI) 2193
RRIDAB_2918697
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y285
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FARSA antibody 67945-1-IgDownload protocol
IHC protocol for FARSA antibody 67945-1-IgDownload protocol
IF protocol for FARSA antibody 67945-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols