FARSB Polyclonal antibody

FARSB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16341-1-AP

Print datasheet

Synonyms

Phenylalanine--tRNA ligase beta subunit, PheHB, HSPC173, FRSB, FARSLB



经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, HL-60 cells, K-562 cells, human liver tissue, mouse brain tissue, PC-3 cells, mouse liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy FARSB Ready-To-Use IHC Kit. FARSB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16341-1-AP targets FARSB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen FARSB fusion protein Ag9522 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phenylalanyl-tRNA synthetase, beta subunit
Synonyms Phenylalanine--tRNA ligase beta subunit, PheHB, HSPC173, FRSB, FARSLB
Calculated Molecular Weight 589 aa, 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC017783
Gene Symbol FARSB
Gene ID (NCBI) 10056
RRIDAB_2102515
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NSD9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FARSB antibody 16341-1-APDownload protocol
IHC protocol for FARSB antibody 16341-1-APDownload protocol
IF protocol for FARSB antibody 16341-1-APDownload protocol
IP protocol for FARSB antibody 16341-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

J Am Chem Soc

Oxidation-Controlled, Strain-Promoted Tellurophene-Alkyne Cycloaddition (OSTAC): A Bioorthogonal Tellurophene-Dependent Conjugation Reaction

Authors - Yong Jia Bu
human,ratWB

Nat Commun

Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.

Authors - Megha Jhanji
ratWB

Neural Regen Res

A novel therapeutic target for peripheral nerve injury-related diseases: aminoacyl-tRNA synthetases.

Authors - Byung Sun Park
human,mouseWB,IF

Nat Commun

Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA

Authors - Mirjana Malnar Črnigoj
human,mouseWB,IHC,IF

Int J Mol Sci

FARSB Facilitates Hepatocellular Carcinoma Progression by Activating the mTORC1 Signaling Pathway

Authors - Yaofeng Wang