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验证数据展示

  • WB analysis of Jurkat using 60196-1-Ig (same clone as 60196-1-PBS)

    WB analysis of Jurkat using 60196-1-Ig (same clone as 60196-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60196-1-Ig (same clone as 60196-1-PBS)

    IHC staining of human tonsillitis using 60196-1-Ig (same clone as 60196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60196-1-Ig (same clone as 60196-1-PBS)

    IHC staining of human tonsillitis using 60196-1-Ig (same clone as 60196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Fas/CD95 Monoclonal antibody, PBS Only

Fas/CD95 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60196-1-PBS

产品说明书

CloneNo. 1G7F7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA

别名

ALPS1A, APO 1, Apo 1 antigen, APT1, CD95, FAS, FAS/CD95, FAS1, FASLG receptor, FASTM, TNFRSF6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60196-1-PBS targets Fas/CD95 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Fas/CD95 fusion protein Ag3718 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Fas (TNF receptor superfamily, member 6)
别名 ALPS1A, APO 1, Apo 1 antigen, APT1, CD95, FAS, FAS/CD95, FAS1, FASLG receptor, FASTM, TNFRSF6
计算分子量 35-38 kDa
观测分子量 38-45 kDa
GenBank蛋白编号BC012479
基因名称 Fas/CD95
Gene ID (NCBI) 355
RRIDAB_10892669
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25445
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). This anti-Fas monoclonal antibody can be used to induce apoptosis in cell cultures through Fas by imitating the Fas-ligand.