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  • Featured Product
  • KD/KO Validated

FASN Monoclonal antibody, PBS Only

FASN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B6E4

Cat no : 66591-1-PBS

Print datasheet

Synonyms

Type I fatty acid synthase, fatty acid synthase, FAS, 1B6E4

验证数据展示

  • WB analysis of HeLa using 66591-1-Ig (same clone as 66591-1-PBS)

    WB analysis of HeLa using 66591-1-Ig (same clone as 66591-1-PBS)

    WB result of FASN antibody (66591-1-Ig; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-FASN transfected HeLa cells. This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66591-1-Ig (same clone as 66591-1-PBS)

    IHC staining of human breast cancer using 66591-1-Ig (same clone as 66591-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66591-1-Ig (FASN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FASN antibody included
  • WB analysis of HepG2 using 66591-1-Ig (same clone as 66591-1-PBS)

    WB analysis of HepG2 using 66591-1-Ig (same clone as 66591-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66591-1-Ig (FASN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66591-1-Ig (same clone as 66591-1-PBS)

    IF Staining of HeLa using 66591-1-Ig (same clone as 66591-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FASN antibody (66591-1-Ig, Clone: 1B6E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy FASN Ready-To-Use IHC Kit. FASN primary antibody included.

产品信息

66591-1-PBS targets FASN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen FASN fusion protein Ag0975 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name fatty acid synthase
Synonyms Type I fatty acid synthase, fatty acid synthase, FAS, 1B6E4
Calculated Molecular Weight 272 kDa
Observed Molecular Weight 272 kDa
GenBank Accession NumberBC007909
Gene Symbol FASN
Gene ID (NCBI) 2194
RRIDAB_2881951
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49327
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.