• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HeLa using 66591-1-Ig (same clone as 66591-1-PBS)

    WB analysis of HeLa using 66591-1-Ig (same clone as 66591-1-PBS)

    WB result of FASN antibody (66591-1-Ig; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-FASN transfected HeLa cells. This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66591-1-Ig (same clone as 66591-1-PBS)

    IHC staining of human breast cancer using 66591-1-Ig (same clone as 66591-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66591-1-Ig (FASN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FASN antibody included
  • WB analysis of HepG2 using 66591-1-Ig (same clone as 66591-1-PBS)

    WB analysis of HepG2 using 66591-1-Ig (same clone as 66591-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66591-1-Ig (FASN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66591-1-Ig (same clone as 66591-1-PBS)

    IF Staining of HeLa using 66591-1-Ig (same clone as 66591-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FASN antibody (66591-1-Ig, Clone: 1B6E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

FASN Monoclonal antibody, PBS Only

FASN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66591-1-PBS

产品说明书

CloneNo. 1B6E4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Type I fatty acid synthase, fatty acid synthase, FAS, 1B6E4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy FASN Ready-To-Use IHC Kit. FASN primary antibody included.

产品信息

66591-1-PBS targets FASN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 FASN fusion protein Ag0975 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 fatty acid synthase
别名 Type I fatty acid synthase, fatty acid synthase, FAS, 1B6E4
计算分子量 272 kDa
观测分子量 272 kDa
GenBank蛋白编号BC007909
基因名称 FASN
Gene ID (NCBI) 2194
RRIDAB_2881951
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP49327
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.