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FASN Monoclonal antibody, PBS Only

FASN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B6E4

Cat no : 66591-1-PBS

Print datasheet

Synonyms

FAS, FASN, fatty acid synthase, OA 519, SDR27X1

验证数据展示

  • WB analysis of HepG2 using 66591-1-Ig (same clone as 66591-1-PBS)

    WB analysis of HepG2 using 66591-1-Ig (same clone as 66591-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66591-1-Ig (FASN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66591-1-Ig (same clone as 66591-1-PBS)

    IHC staining of human breast cancer using 66591-1-Ig (same clone as 66591-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66591-1-Ig (FASN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FASN antibody included
  • IF Staining of HeLa using 66591-1-Ig (same clone as 66591-1-PBS)

    IF Staining of HeLa using 66591-1-Ig (same clone as 66591-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FASN antibody (66591-1-Ig, Clone: 1B6E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy FASN Ready-To-Use IHC Kit. FASN primary antibody included.

产品信息

66591-1-PBS targets FASN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen FASN fusion protein Ag0975 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name fatty acid synthase
Synonyms FAS, FASN, fatty acid synthase, OA 519, SDR27X1
Calculated Molecular Weight 272 kDa
Observed Molecular Weight 272 kDa
GenBank Accession NumberBC007909
Gene Symbol FASN
Gene ID (NCBI) 2194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49327
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.