• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HeLa using 81079-1-RR

    IF Staining of HeLa using 81079-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FASN antibody (81079-1-RR, Clone: 1L9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 81079-1-RR

    WB analysis using 81079-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81079-1-RR (FASN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 81079-1-RR

    Affinity and Kinetic Characterization of 81079-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81079-1-RR against Human FASN were performed. The affinity constant is below 1 pM.

FASN Recombinant antibody

FASN Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 81079-1-RR

产品说明书

CloneNo. 1L9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

3-oxoacyl-[acyl-carrier-protein] synthase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, 1L9, [Acyl-carrier-protein] S-malonyltransferase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, U-251 cells, Neuro-2a cells, HSC-T6 cells, mouse liver tissue, rat liver tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy FASN Ready-To-Use IHC Kit. FASN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81079-1-RR targets FASN in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FASN fusion protein Ag0975 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fatty acid synthase
别名 3-oxoacyl-[acyl-carrier-protein] synthase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, 1L9, [Acyl-carrier-protein] S-malonyltransferase
计算分子量 272 kDa
观测分子量250-270 kDa
GenBank蛋白编号BC007909
基因名称 FASN
Gene ID (NCBI) 2194
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49327
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.

实验方案

Product Specific Protocols
WB protocol for FASN antibody 81079-1-RRDownload protocol
IF protocol for FASN antibody 81079-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols