FASN Recombinant antibody
FASN Recombinant Antibody for IF/ICC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
IF/ICC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1L9
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, MCF-7 cells, U-251 cells, Neuro-2a cells, HSC-T6 cells, mouse liver tissue, rat liver tissue |
Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy FASN Ready-To-Use IHC Kit. FASN primary antibody included.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF) | IF : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
81079-1-RR targets FASN in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | IF/ICC, WB, ELISA Application Description |
Tested Reactivity | Human, mouse, rat |
Immunogen | FASN fusion protein Ag0975 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | fatty acid synthase |
Synonyms | FAS, FASN, fatty acid synthase, OA 519, SDR27X1 |
Calculated Molecular Weight | 272 kDa |
Observed Molecular Weight | 250-270 kDa |
GenBank Accession Number | BC007909 |
Gene Symbol | FASN |
Gene ID (NCBI) | 2194 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P49327 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.
实验方案
Product Specific Protocols | |
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WB protocol for FASN antibody 81079-1-RR | Download protocol |
IF protocol for FASN antibody 81079-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |