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验证数据展示

  • WB analysis of HeLa using 18392-1-AP

    WB analysis of HeLa using 18392-1-AP

    WB result of FASTKD3 antibody (18392-1-AP, 1:200) with HeLa and 293 cell lysate by Maciej Szewczyk University of Warsaw.

  • IF Staining of transfected cells using 18392-1-AP

    IF Staining of transfected cells using 18392-1-AP

    IF result of anti-FASTKD3 (18392-1-AP, 1:200) with transfected COS7 cell by Dr. Nancy Kedersha.

  • WB analysis of HepG2 using 18392-1-AP

    WB analysis of HepG2 using 18392-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18392-1-AP (FASTKD3 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • IHC staining of human breast cancer using 18392-1-AP

    IHC staining of human breast cancer using 18392-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18392-1-AP (FASTKD3 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 18392-1-AP

    IHC staining of human breast cancer using 18392-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18392-1-AP (FASTKD3 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

FASTKD3 Polyclonal antibody

FASTKD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18392-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

FAST kinase domain-containing protein 3, mitochondrial

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected intransfected cells, HeLa cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18392-1-AP targets FASTKD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 FASTKD3 fusion protein Ag13083 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FAST kinase domains 3
别名 FAST kinase domain-containing protein 3, mitochondrial
计算分子量 76 kDa
观测分子量 70 kDa
GenBank蛋白编号BC001295
基因名称 FASTKD3
Gene ID (NCBI) 79072
RRIDAB_2878541
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14CZ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAST kinase domain-containing protein 3(FASTKD3) belongs to FASTKD family.FASTKD3 is an essential component of mitochondrial respiration that may modulate energy balance in cells exposed to adverse conditions by functionally coupling mitochondrial protein synthesis to respiration. The expression of endogenous FASTKD3 is very weak. This antibody detects the c-terminal of FASTKD3.

实验方案

Product Specific Protocols
WB protocol for FASTKD3 antibody 18392-1-APDownload protocol
IHC protocol for FASTKD3 antibody 18392-1-APDownload protocol
IF protocol for FASTKD3 antibody 18392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

Role of FAST kinase domains 3 (FASTKD3) in post-transcriptional regulation of mitochondrial gene expression.

Authors - Erik Boehm
  • KO Validated
View Article