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  • Featured Product
  • KD/KO Validated

FASTKD3 Polyclonal antibody

FASTKD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18392-1-AP

Print datasheet

Synonyms

FAST kinase domain-containing protein 3, mitochondrial

验证数据展示

  • WB analysis of HeLa using 18392-1-AP

    WB analysis of HeLa using 18392-1-AP

    WB result of FASTKD3 antibody (18392-1-AP, 1:200) with HeLa and 293 cell lysate by Maciej Szewczyk University of Warsaw.

  • IF Staining of transfected cells using 18392-1-AP

    IF Staining of transfected cells using 18392-1-AP

    IF result of anti-FASTKD3 (18392-1-AP, 1:200) with transfected COS7 cell by Dr. Nancy Kedersha.

  • WB analysis of HepG2 using 18392-1-AP

    WB analysis of HepG2 using 18392-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18392-1-AP (FASTKD3 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • IHC staining of human breast cancer using 18392-1-AP

    IHC staining of human breast cancer using 18392-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18392-1-AP (FASTKD3 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 18392-1-AP

    IHC staining of human breast cancer using 18392-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18392-1-AP (FASTKD3 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected intransfected cells, HeLa cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18392-1-AP targets FASTKD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen FASTKD3 fusion protein Ag13083 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FAST kinase domains 3
Synonyms FAST kinase domain-containing protein 3, mitochondrial
Calculated Molecular Weight 76 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC001295
Gene Symbol FASTKD3
Gene ID (NCBI) 79072
RRIDAB_2878541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14CZ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAST kinase domain-containing protein 3(FASTKD3) belongs to FASTKD family.FASTKD3 is an essential component of mitochondrial respiration that may modulate energy balance in cells exposed to adverse conditions by functionally coupling mitochondrial protein synthesis to respiration. The expression of endogenous FASTKD3 is very weak. This antibody detects the c-terminal of FASTKD3.

实验方案

Product Specific Protocols
WB protocol for FASTKD3 antibody 18392-1-APDownload protocol
IHC protocol for FASTKD3 antibody 18392-1-APDownload protocol
IF protocol for FASTKD3 antibody 18392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

Role of FAST kinase domains 3 (FASTKD3) in post-transcriptional regulation of mitochondrial gene expression.

Authors - Erik Boehm
  • KO Validated
View Article