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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 30733-1-AP (same clone as 30733-1-PBS)

    WB analysis using 30733-1-AP (same clone as 30733-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30733-1-AP (FAT1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30733-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate hyperplasia using 30733-1-AP (same clone as 30733-1-PBS)

    IHC staining of human prostate hyperplasia using 30733-1-AP (same clone as 30733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 30733-1-AP (FAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30733-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate hyperplasia using 30733-1-AP (same clone as 30733-1-PBS)

    IHC staining of human prostate hyperplasia using 30733-1-AP (same clone as 30733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 30733-1-AP (FAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30733-1-PBS in a different storage buffer formulation.

FAT1 Polyclonal antibody, PBS Only

FAT1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30733-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

30733-1-PBS targets FAT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 FAT1 fusion protein Ag33957 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FAT tumor suppressor homolog 1 (Drosophila)
别名
观测分子量506-600 kDa
基因名称 FAT1
Gene ID (NCBI) 2195
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14517
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FAT1, also known as hFat1, belongs to a member of the cadherin superfamily, has been proposed to play roles in cerebral development, glomerular slit formation, and also to act as a tumor suppressor, but its mechanisms of action have not been elucidated. It is expected to be located in cell membrane and nucleus, which is expressed in many epithelial and some endothelial and smooth muscle cells. The calculated molecular weight of FAT1 is 506 kDa and there is glycosylation modification of the protein. To examine functions of the transmembrane and cytoplasmic domains, they were expressed in HEK-293 and HeLa cells as chimeric proteins in fusion with EGFP and extracellular domains derived from E-cadherin. Proteins comprising the transmembrane domain localized to the membrane fraction (PMID: 15922730, 26373379).