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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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验证数据展示

  • WB analysis using 31490-1-AP

    WB analysis using 31490-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31490-1-AP (FAT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

FAT2 Polyclonal antibody

FAT2 Polyclonal Antibody for WB, ELISA
Cat No. 31490-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Protocadherin Fat 2, MEGF1, KIAA0811, HFAT2, CDHF8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31490-1-AP targets FAT2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 FAT2 fusion protein Ag34942 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FAT tumor suppressor homolog 2 (Drosophila)
别名 Protocadherin Fat 2, MEGF1, KIAA0811, HFAT2, CDHF8
计算分子量479 kDa
观测分子量500-600 kDa
GenBank蛋白编号NM_001447
基因名称 FAT2
Gene ID (NCBI) 2196
RRIDAB_3670004
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9NYQ8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAT2 (FAT atypical cadherin 2), also known as CDHF8. The gene product is a member of the cadherin superfamily, a group of integral membrane proteins characterized by the presence of cadherin-type repeats. This protein most likely functions as a cell adhesion molecule, controlling cell proliferation and playing an important role in cerebellum development. Expressed in epidermal keratinocytes, infant brain, cerebellum, and also in a variety of tumors, such as pancreatic cancer, diffuse type gastric cancer, ovarian cancer, esophageal cancer, skin squamous cell carcinoma, head and neck cancer. Not expressed in melanoma cell line A375 cells, normal epidermal melanocytes or normal dermal fibroblasts. Expressed in epidermal keratinocytes and squamous cell carcinoma (at protein level). The molecular weight of FAT2 is 473 kDa. The huge molecular masses (about 500-600 kDa) have hindered studies of the molecular aspects of itself roles(PMID: 15923647).

实验方案

Product Specific Protocols
WB protocol for FAT2 antibody 31490-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols