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FATP5/SLC27A5 Polyclonal antibody

FATP5/SLC27A5 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25347-1-AP

Print datasheet

Synonyms

FATP5, SLC27A5, Cholate--CoA ligase, Bile acyl-CoA synthetase, Bile acid-CoA ligase

验证数据展示

  • WB analysis using 25347-1-AP

    WB analysis using 25347-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25347-1-AP (FATP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of rat liver using 25347-1-AP

    IP experiment of rat liver using 25347-1-AP

    IP result of anti-FATP5 (IP:25347-1-AP, 4ug; Detection:25347-1-AP 1:5000) with rat liver tissue lysate 2695 ug.

经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IP detected inrat liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25347-1-AP targets FATP5/SLC27A5 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen FATP5/SLC27A5 fusion protein Ag18446 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 27 (fatty acid transporter), member 5
Synonyms FATP5, SLC27A5, Cholate--CoA ligase, Bile acyl-CoA synthetase, Bile acid-CoA ligase
Calculated Molecular Weight 690 aa, 75 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC148807
Gene Symbol FATP5/SLC27A5
Gene ID (NCBI) 10998
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2P5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC27A5, also known as FATP5, ACSVL6, and BAL, is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty acids containing 24- and 26-carbons(PMID: 10479480). FATP5 mediates the import of long-chain fatty acids (LCFA) by facilitating their transport across cell membranes (PubMed:20448275). FATP5 is associated with endoplasmic reticulum but not with peroxisomes, and its primary role is in fatty acid elongation or complex lipid synthesis. FATP5 is exclusively expressed by the liver and localized to the basal plasma membrane of hepatocytes (PMID:16618416).

实验方案

Product Specific Protocols
WB protocol for FATP5/SLC27A5 antibody 25347-1-APDownload protocol
IP protocol for FATP5/SLC27A5 antibody 25347-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Front Oncol

FATP5 modulates biological activity and lipid metabolism in prostate cancer through the TEAD4-mediated Hippo signaling

Authors - Shenyang Liu
View Article