验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IP detected in | rat liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
25347-1-AP targets FATP5/SLC27A5 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | FATP5/SLC27A5 fusion protein Ag18446 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 27 (fatty acid transporter), member 5 |
| 别名 | FATP5, SLC27A5, Cholate--CoA ligase, Bile acyl-CoA synthetase, Bile acid-CoA ligase |
| 计算分子量 | 690 aa, 75 kDa |
| 观测分子量 | 70-75 kDa |
| GenBank蛋白编号 | BC148807 |
| 基因名称 | FATP5/SLC27A5 |
| Gene ID (NCBI) | 10998 |
| RRID | AB_3669470 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y2P5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC27A5, also known as FATP5, ACSVL6, and BAL, is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty acids containing 24- and 26-carbons(PMID: 10479480). FATP5 mediates the import of long-chain fatty acids (LCFA) by facilitating their transport across cell membranes (PubMed:20448275). FATP5 is associated with endoplasmic reticulum but not with peroxisomes, and its primary role is in fatty acid elongation or complex lipid synthesis. FATP5 is exclusively expressed by the liver and localized to the basal plasma membrane of hepatocytes (PMID:16618416).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FATP5/SLC27A5 antibody 25347-1-AP | Download protocol |
| IP protocol for FATP5/SLC27A5 antibody 25347-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |