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验证数据展示

  • WB analysis of mouse brain using 11394-1-AP

    WB analysis of mouse brain using 11394-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11394-1-AP (FBF1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 11394-1-AP

    IP experiment of mouse brain using 11394-1-AP

    IP result of anti-FBF1 (IP:11394-1-AP, 4ug; Detection:11394-1-AP 1:400) with mouse brain tissue lysate 5000ug.

FBF1 Polyclonal antibody

FBF1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 11394-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

Alb, albatross, Fas (TNFRSF6) binding factor 1, FBF 1, FBF1, FBF1 Ens, HCG1989313, isoform CRA_b, KIAA1863

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11394-1-AP targets FBF1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FBF1 fusion protein Ag1954 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Fas (TNFRSF6) binding factor 1
别名 Alb, albatross, Fas (TNFRSF6) binding factor 1, FBF 1, FBF1, FBF1 Ens, HCG1989313, isoform CRA_b, KIAA1863
计算分子量1133 aa, 125 kDa
观测分子量 69-70 kDa
GenBank蛋白编号BC012332
基因名称 FBF1
Gene ID (NCBI) 85302
RRIDAB_10638320
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TES7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by the fragment (492-792aa) of FBF1. It recognizes isoform 1, 2, 3, 5 and 6.

实验方案

Product Specific Protocols
WB protocol for FBF1 antibody 11394-1-APDownload protocol
IP protocol for FBF1 antibody 11394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols