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Fibulin 5 Polyclonal antibody

Fibulin 5 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 12188-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (1)

ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, UP50, Urine p50 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse heart tissue, rat brain tissue, mouse placenta tissue, mouse skeletal muscle tissue, mouse kidney tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse lung tissue, human heart tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12188-1-AP targets Fibulin 5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 Fibulin 5 fusion protein Ag2859 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fibulin 5
别名 ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, UP50, Urine p50 protein
计算分子量 448 aa, 50 kDa
观测分子量55 kDa, 65 kDa
GenBank蛋白编号BC022280
基因名称 Fibulin 5
Gene ID (NCBI) 10516
RRIDAB_2105939
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBX5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies. This antibody got 50 kDa or 66 kDa band in western blotting, the 66 kDa corresponding to fully glycosylated protein.

实验方案

Product Specific Protocols
WB protocol for Fibulin 5 antibody 12188-1-APDownload protocol
IHC protocol for Fibulin 5 antibody 12188-1-APDownload protocol
IP protocol for Fibulin 5 antibody 12188-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Arterioscler Thromb Vasc Biol

Increased aortic stiffness and attenuated lysyl oxidase activity in obesity.

Authors - Chen Ju-Yi JY
humanWB

Biochem J

Fibulin-5 binds urokinase-type plasminogen activator and mediates urokinase-stimulated β1-integrin-dependent cell migration.

Authors - Kapustin Alexander A
mouseWB, IHC

Cell Tissue Res

Aberrant elastin remodeling in the lungs of O2-exposed newborn mice; primarily results from perturbed interaction between integrins and elastin.

Authors - Wenli Han
mouseWB

Am J Physiol Cell Physiol

Caveolin-1 stabilizes ATP7A, Cu transporter for Extracellular SOD, in Vascular Tissue to Maintain Endothelial Function.

Authors - Varadarajan Sudhahar
humanWB

Clin Exp Pharmacol Physiol

Limb remote ischemic postconditioning-induced elevation of fibulin-5 confers neuroprotection to rats with cerebral ischemia/reperfusion injury: activation of the AKT pathway.

Authors - Wei Zhang
  • KD Validated
humanIF

JID Innov

Fibulin-4 Accelerates Amyloid Formation by Binding with a Keratin 5 Peptide Fragment.

Authors - Fumihiko Katagiri
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