验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, HepG2 cells, L02 cells, mouse lung tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 2 publications below |
产品信息
20252-1-AP targets fibrillin 2-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fibrillin 2 |
| 别名 | CCA, DA9, FBN2, fibrillin 2, fibrillin 2-Specific |
| 计算分子量 | 315 kDa |
| 观测分子量 | 290 kDa, 160 kDa |
| GenBank蛋白编号 | NM_001999 |
| 基因名称 | Fibrillin 2 |
| Gene ID (NCBI) | 2201 |
| RRID | AB_11182265 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35556 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FBN2 belongs to the fibrillin family. Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils which occur either in association with elastin or in elastin-free bundles. Fibrillin-2-containing microfibrils regulate the early process of elastic fiber assembly. Defects in FBN2 are the cause of congenital contractural arachnodactyly (CCA) which also known as Beals syndrome or distal arthrogryposis type 9 (DA9). This antibody is specific to FBN2. It recognizes both two isoforms ~300 kDa and 160 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for fibrillin 2-Specific antibody 20252-1-AP | Download protocol |
| IHC protocol for fibrillin 2-Specific antibody 20252-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Head Neck High expression of NFX1-123 in HPV positive head and neck squamous cell carcinomas. | ||
J Ovarian Res hUMSC transplantation restores follicle development in ovary damaged mice via re-establish extracellular matrix (ECM) components | ||
J Ethnopharmacol Therapeutic effects of Mudan Granules on diabetic retinopathy: mitigating fibrogenesis caused by FBN2 deficiency and inflammation associated with TNF-α elevation |
