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FBP1 Monoclonal antibody, PBS Only

FBP1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G4E4

Cat No : 68446-1-PBS

Print datasheet

Synonyms

FBP, FBP1, FBPase 1, fructose 1,6 bisphosphatase 1

验证数据展示

  • WB analysis using 68446-1-Ig (same clone as 68446-1-PBS)

    WB analysis using 68446-1-Ig (same clone as 68446-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 68446-1-Ig (same clone as 68446-1-PBS)

    IF Staining of MCF-7 using 68446-1-Ig (same clone as 68446-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FBP1 antibody (68446-1-Ig, Clone: 2G4E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 68446-1-Ig (same clone as 68446-1-PBS)

    IHC staining of mouse kidney using 68446-1-Ig (same clone as 68446-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FBP1 antibody included
  • IHC staining of mouse kidney using 68446-1-Ig (same clone as 68446-1-PBS)

    IHC staining of mouse kidney using 68446-1-Ig (same clone as 68446-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FBP1 antibody included
Planning an IHC experiment? We recommend our IHCeasy FBP1 Ready-To-Use IHC Kit. FBP1 primary antibody included.

产品信息

68446-1-PBS targets FBP1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen FBP1 fusion protein Ag3837 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name fructose-1,6-bisphosphatase 1
Synonyms FBP, FBP1, FBPase 1, fructose 1,6 bisphosphatase 1
Calculated Molecular Weight 338 aa, 37 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC012927
Gene Symbol FBP1
Gene ID (NCBI) 2203
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09467
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

FBP1 (Fructose-1,6-bisphosphatase 1) is also named as FBP and belongs to the FBPase class 1 family. It catalyzes the hydrolysis of fructose-1,6 bisphosphate to fructose-6-phosphate and inorganic phosphate. This reaction is an important regulatory site of gluconeogenesis. Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) (PMID:12126934).