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验证数据展示

  • WB analysis of mouse brain using 27056-1-AP

    WB analysis of mouse brain using 27056-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 27056-1-AP (FBP17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 27056-1-AP

    IP experiment of mouse brain using 27056-1-AP

    IP result of anti-FBP17 (IP:27056-1-AP, 4ug; Detection:27056-1-AP 1:1000) with mouse brain tissue lysate 3000ug.

  • IHC staining of human lung cancer using 27056-1-AP

    IHC staining of human lung cancer using 27056-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human lung cancer using 27056-1-AP

    IHC staining of human lung cancer using 27056-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 40x lens).

FBP17 Polyclonal antibody

FBP17 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 27056-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

FBP17, FNBP1, formin binding protein 1, Formin binding protein 17, hFBP17, KIAA0554

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:150-1:600
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27056-1-AP targets FBP17 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 FBP17 fusion protein Ag25784 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 formin binding protein 1
别名 FBP17, FNBP1, formin binding protein 1, Formin binding protein 17, hFBP17, KIAA0554
观测分子量 80-85 kDa
GenBank蛋白编号BC101755
基因名称 FBP17
Gene ID (NCBI) 23048
RRIDAB_2880734
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RU3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBP17 (formin-binding protein 17) is a new dynamin-associating molecule consisting of several functional domains, including an N-terminal EFC domain and an SH3 domain at the C-terminus. FBP17 is involved in dynamin-mediated endocytosis and has been reported to be required for invadopodia formation and tumor cell invasion.

实验方案

Product Specific Protocols
WB protocol for FBP17 antibody 27056-1-APDownload protocol
IHC protocol for FBP17 antibody 27056-1-APDownload protocol
IP protocol for FBP17 antibody 27056-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

bioRxiv

F-BAR proteins CIP4 and FBP17 function in cortical neuron radial migration and process outgrowth

Authors - Lauren A English
View Article