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FBP17 Polyclonal antibody

FBP17 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27056-1-AP

Print datasheet

Synonyms

FBP17, FNBP1, formin binding protein 1, Formin binding protein 17, hFBP17, KIAA0554

验证数据展示

  • WB analysis of mouse brain using 27056-1-AP

    WB analysis of mouse brain using 27056-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 27056-1-AP (FBP17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 27056-1-AP

    IP experiment of mouse brain using 27056-1-AP

    IP result of anti-FBP17 (IP:27056-1-AP, 4ug; Detection:27056-1-AP 1:1000) with mouse brain tissue lysate 3000ug.

  • IHC staining of human lung cancer using 27056-1-AP

    IHC staining of human lung cancer using 27056-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human lung cancer using 27056-1-AP

    IHC staining of human lung cancer using 27056-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:150-1:600
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 27056-1-AP is FBP17 Fusion Protein expressed in E. coli.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen FBP17 fusion protein Ag25784 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name formin binding protein 1
Synonyms FBP17, FNBP1, formin binding protein 1, Formin binding protein 17, hFBP17, KIAA0554
Observed Molecular Weight 80-85 kDa
GenBank Accession NumberBC101755
Gene Symbol FBP17
Gene ID (NCBI) 23048
RRIDAB_2880734
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RU3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBP17 (formin-binding protein 17) is a new dynamin-associating molecule consisting of several functional domains, including an N-terminal EFC domain and an SH3 domain at the C-terminus. FBP17 is involved in dynamin-mediated endocytosis and has been reported to be required for invadopodia formation and tumor cell invasion.

实验方案

Product Specific Protocols
WB protocol for FBP17 antibody 27056-1-APDownload protocol
IHC protocol for FBP17 antibody 27056-1-APDownload protocol
IP protocol for FBP17 antibody 27056-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols