验证数据展示
经过测试的应用
Positive WB detected in | MDA-MB-453s cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20895-1-AP targets FBXL15 in WB, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human |
免疫原 | FBXL15 fusion protein Ag14949 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | F-box and leucine-rich repeat protein 15 |
别名 | F box only protein 37, F box/LRR repeat protein 15, Fbl15, FBXL15, FBXO37, JET, PSD |
计算分子量 | 300 aa, 33 kDa |
观测分子量 | 33 kDa |
GenBank蛋白编号 | BC002912 |
基因名称 | FBXL15 |
Gene ID (NCBI) | 79176 |
RRID | AB_2878758 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9H469 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FBXL15, also known as FBXO37, belongs to the FBXL15 family. FBXL15 plays a role in the G2/M transition of the cell cycle, as well as in cellular protein metabolic processes and the positive regulation of the bone morphogenetic proteins signaling pathway (PMID: 38711244). The calculated molecular weight of FBXL15 is 33 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for FBXL15 antibody 20895-1-AP | Download protocol |
IHC protocol for FBXL15 antibody 20895-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |