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验证数据展示

  • IHC staining of human breast cancer using 20895-1-AP

    IHC staining of human breast cancer using 20895-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20895-1-AP (FBXL15 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 20895-1-AP

    IHC staining of human breast cancer using 20895-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20895-1-AP (FBXL15 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of MDA-MB-453s using 20895-1-AP

    WB analysis of MDA-MB-453s using 20895-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20895-1-AP (FBXL15 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

FBXL15 Polyclonal antibody

FBXL15 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20895-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

F box only protein 37, F box/LRR repeat protein 15, Fbl15, FBXL15, FBXO37, JET, PSD

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMDA-MB-453s cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20895-1-AP targets FBXL15 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen FBXL15 fusion protein Ag14949 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name F-box and leucine-rich repeat protein 15
Synonyms F box only protein 37, F box/LRR repeat protein 15, Fbl15, FBXL15, FBXO37, JET, PSD
Calculated Molecular Weight 300 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC002912
Gene Symbol FBXL15
Gene ID (NCBI) 79176
RRIDAB_2878758
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H469
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXL15, also known as FBXO37, belongs to the FBXL15 family. FBXL15 plays a role in the G2/M transition of the cell cycle, as well as in cellular protein metabolic processes and the positive regulation of the bone morphogenetic proteins signaling pathway (PMID: 38711244). The calculated molecular weight of FBXL15 is 33 kDa.

实验方案

Product Specific Protocols
WB protocol for FBXL15 antibody 20895-1-APDownload protocol
IHC protocol for FBXL15 antibody 20895-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols