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FBXL16 Polyclonal antibody

FBXL16 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25950-1-AP

Print datasheet

Synonyms

C16orf22, c380A1.1, F box/LRR repeat protein 16, Fbl16, FBXL16

验证数据展示

  • WB analysis of HeLa using 25950-1-AP

    WB analysis of HeLa using 25950-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25950-1-AP (FBXL16 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 25950-1-AP

    IHC staining of human brain using 25950-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25950-1-AP (FBXL16 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 25950-1-AP

    IHC staining of human brain using 25950-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25950-1-AP (FBXL16 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

25950-1-AP targets FBXL16 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen FBXL16 fusion protein Ag22827 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name F-box and leucine-rich repeat protein 16
Synonyms C16orf22, c380A1.1, F box/LRR repeat protein 16, Fbl16, FBXL16
Calculated Molecular Weight 479 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC036680
Gene Symbol FBXL16
Gene ID (NCBI) 146330
RRIDAB_2880307
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N461
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FBXL16 antibody 25950-1-APDownload protocol
IHC protocol for FBXL16 antibody 25950-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Nat Chem Biol

LATS2 condensates organize signalosomes for Hippo pathway signal transduction

Authors - Min Qin
View Article