• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of NCI-H1299 using 68246-1-Ig

    WB analysis of NCI-H1299 using 68246-1-Ig

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 68246-1-Ig (FBXO17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68246-1-Ig

    IF Staining of HepG2 using 68246-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FBXO17 antibody (68246-1-Ig, Clone: 1F3D3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

FBXO17 Monoclonal antibody

FBXO17 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68246-1-Ig

Print datasheet

CloneNo. 1F3D3

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

F box only protein 17, F box only protein 26, F box protein 17, FBG4, Fbx17, FBX26, FBXO17, FBXO26, FLJ11798, FLJ25205, FLJ58300

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488

Size: 
50 μL
100 μL
150 μL

¥2200/ 100 μL

浓度: 1000 μg/ml

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inNCI-H1299 cells, U2OS cells, A549 cells, SH-SY5Y cells, U-87 MG cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68246-1-Ig targets FBXO17 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen FBXO17 fusion protein Ag3867 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name F-box protein 17
Synonyms F box only protein 17, F box only protein 26, F box protein 17, FBG4, Fbx17, FBX26, FBXO17, FBXO26, FLJ11798, FLJ25205, FLJ58300
Calculated Molecular Weight 278 aa, 31 kDa
Observed Molecular Weight29-31 kDa
GenBank Accession NumberBC012385
Gene Symbol FBXO17
Gene ID (NCBI) 115290
RRIDAB_2935332
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96EF6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXO17, also named as FBG4, FBX17, FBX26 and FBXO26, is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.

实验方案

Product Specific Protocols
WB protocol for FBXO17 antibody 68246-1-IgDownload protocol
IF protocol for FBXO17 antibody 68246-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols