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FBXO18 Polyclonal antibody

FBXO18 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14275-1-AP

Print datasheet

Synonyms

FBX18, F-box only protein 18, F-box DNA helicase 1, FBH1, EC:5.6.2.4

验证数据展示

  • WB analysis of PC-3 using 14275-1-AP

    WB analysis of PC-3 using 14275-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14275-1-AP (FBXO18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 14275-1-AP

    IF Staining of PC-3 using 14275-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 14275-1-AP (FBXO18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inPC-3 cells, HeLa cells
Positive IF/ICC detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14275-1-AP targets FBXO18 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen FBXO18 fusion protein Ag5622 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name F-box protein, helicase, 18
Synonyms FBX18, F-box only protein 18, F-box DNA helicase 1, FBH1, EC:5.6.2.4
Calculated Molecular Weight 118 kDa, 123 kDa
Observed Molecular Weight 118-123 kDa
GenBank Accession NumberBC032674
Gene Symbol FBXO18
Gene ID (NCBI) 84893
RRIDAB_10638916
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFZ0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FBXO18 antibody 14275-1-APDownload protocol
IF protocol for FBXO18 antibody 14275-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols