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  • Featured Product
  • KD/KO Validated

FBXO27 Polyclonal antibody

FBXO27 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14570-1-AP

Print datasheet

Synonyms

FBX27, F-box/G-domain protein 5, F-box only protein 27, FBG5

验证数据展示

  • IP experiment of PANC-1 using 14570-1-AP

    IP experiment of PANC-1 using 14570-1-AP

    IP result of anti-FBXO27 (IP:14570-1-AP, 4ug; Detection:14570-1-AP 1:600) with PANC-1 cells lysate 1330 ug.

  • WB analysis of Panc-1 cells using 14570-1-AP

    WB analysis of Panc-1 cells using 14570-1-AP

    FBXO27 expression in cells. Each whole cell lysate containing 15 ug protein was analyzed by immunoblotting using FBXO27 (14570-1-AP: 1:2000). Knock-down experiment confirmed that the pointed band showed FBXO27. (Data from Dr. Yukiko Yoshida, Ubiquitin Project, Tokyo Metropolitan Institute of Medical Science.).

  • IHC staining of human kidney using 14570-1-AP

    IHC staining of human kidney using 14570-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14570-1-AP (FBXO27 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 14570-1-AP

    IHC staining of human kidney using 14570-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14570-1-AP (FBXO27 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inPANC-1 cells
Positive IP detected inPANC-1 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14570-1-AP targets FBXO27 in WB, IHC, IP, ELISA, IF applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen FBXO27 fusion protein Ag6157 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name F-box protein 27
Synonyms FBX27, F-box/G-domain protein 5, F-box only protein 27, FBG5
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC030060
Gene Symbol FBXO27
Gene ID (NCBI) 126433
RRIDAB_2104422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NI29
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXO27 is a glycoprotein-specific F-box protein that is part of the SCF (SKP1/CUL1/F-box protein) ubiquitin ligase complex, and SCFFBXO27 ubiquitinates glycoproteins in damaged lysosomes will regulate autophagic machinery recruitment (PMID: 28743755).Unlike F-box proteins in other SCF complexes, FBXO27 is subject to N-myristoylation, which localizes it to membranes, allowing it to accumulate rapidly around damaged lysosomes.

实验方案

Product Specific Protocols
IHC protocol for FBXO27 antibody 14570-1-APDownload protocol
IP protocol for FBXO27 antibody 14570-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Proc Natl Acad Sci U S A

Ubiquitination of exposed glycoproteins by SCF(FBXO27) directs damaged lysosomes for autophagy.

Authors - Yukiko Yoshida
  • KO Validated
View Article
humanWB,IF

Autophagy

TRIM16-mediated lysophagy suppresses high-glucose-accumulated neuronal Aβ

Authors - Chang Woo Chae
View Article