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验证数据展示

  • IHC staining of mouse skeletal muscle using 28541-1-AP

    IHC staining of mouse skeletal muscle using 28541-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28541-1-AP (FBXO32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FBXO32 antibody included

FBXO32 Polyclonal antibody

FBXO32 Polyclonal Antibody for IHC, ELISA
Cat No. 28541-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

IHC, ELISA

别名

Atrogin 1, F box only protein 32, F box protein 32, Fbx32, FBXO32, FLJ32424, MAFbx, Muscle atrophy F box protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FBXO32 Ready-To-Use IHC Kit. FBXO32 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28541-1-AP targets FBXO32 in IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 FBXO32 fusion protein Ag29410 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 F-box protein 32
别名 Atrogin 1, F box only protein 32, F box protein 32, Fbx32, FBXO32, FLJ32424, MAFbx, Muscle atrophy F box protein
计算分子量 355 aa, 42 kDa
GenBank蛋白编号BC024030
基因名称 FBXO32
Gene ID (NCBI) 114907
RRIDAB_3086062
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ969P5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXO32 (F box only protein 32), also known as Atrogin 1 or MAFbx, is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif F-box. This protein is an E3 ubiquitin ligase that is markedly up-regulated in muscle atrophy. FBXO32 is thus a potential drug target for the treatment of muscle atrophy. Some data support that FBXO32 may play an important role in tumorigenesis. Recent study reveal that FBXO32 targets the oncogenic protein c-Myc for ubiquitination and degradation through the proteasome pathway.

实验方案

Product Specific Protocols
IHC protocol for FBXO32 antibody 28541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols