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FBXO32 Monoclonal antibody, PBS Only

FBXO32 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Rat, mouse

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

3C2G3

Cat No : 67172-1-PBS

Print datasheet

Synonyms

Atrogin 1, F box only protein 32, F box protein 32, Fbx32, FBXO32, FLJ32424, MAFbx, Muscle atrophy F box protein

验证数据展示

  • WB analysis using 67172-1-Ig (same clone as 67172-1-PBS)

    WB analysis using 67172-1-Ig (same clone as 67172-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    IHC staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FBXO32 antibody included
  • IF Staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    IF Staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using FBXO32 antibody (67172-1-Ig, Clone: 3C2G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy FBXO32 Ready-To-Use IHC Kit. FBXO32 primary antibody included.

产品信息

67172-1-PBS targets FBXO32 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Pig, Rat, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Pig, Rat, mouse
Immunogen FBXO32 fusion protein Ag25247 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name F-box protein 32
Synonyms Atrogin 1, F box only protein 32, F box protein 32, Fbx32, FBXO32, FLJ32424, MAFbx, Muscle atrophy F box protein
Calculated Molecular Weight 355 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC024030
Gene Symbol FBXO32
Gene ID (NCBI) 114907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ969P5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

FBXO32 (F box only protein 32), also known as Atrogin 1 or MAFbx, is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif F-box. This protein is an E3 ubiquitin ligase that is markedly up-regulated in muscle atrophy. FBXO32 is thus a potential drug target for the treatment of muscle atrophy. Some data support that FBXO32 may play an important role in tumorigenesis. Recent study reveal that FBXO32 targets the oncogenic protein c-Myc for ubiquitination and degradation through the proteasome pathway.