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FBXO32 Monoclonal antibody, PBS Only

FBXO32 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Rat, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C2G3

Cat no : 67172-1-PBS

Print datasheet

Synonyms

Atrogin 1, F box only protein 32, F box protein 32, Fbx32, FBXO32, FLJ32424, MAFbx, Muscle atrophy F box protein

验证数据展示

  • WB analysis using 67172-1-Ig (same clone as 67172-1-PBS)

    WB analysis using 67172-1-Ig (same clone as 67172-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    IHC staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FBXO32 antibody included
  • IF Staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    IF Staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using FBXO32 antibody (67172-1-Ig, Clone: 3C2G3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy FBXO32 Ready-To-Use IHC Kit. FBXO32 primary antibody included.

产品信息

67172-1-PBS targets FBXO32 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Pig, Rat, mouse samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, Pig, Rat, mouse
Immunogen FBXO32 fusion protein Ag25247 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name F-box protein 32
Synonyms Atrogin 1, F box only protein 32, F box protein 32, Fbx32, FBXO32, FLJ32424, MAFbx, Muscle atrophy F box protein
Calculated Molecular Weight 355 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC024030
Gene Symbol FBXO32
Gene ID (NCBI) 114907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ969P5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FBXO32 (F box only protein 32), also known as Atrogin 1 or MAFbx, is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif F-box. This protein is an E3 ubiquitin ligase that is markedly up-regulated in muscle atrophy. FBXO32 is thus a potential drug target for the treatment of muscle atrophy. Some data support that FBXO32 may play an important role in tumorigenesis. Recent study reveal that FBXO32 targets the oncogenic protein c-Myc for ubiquitination and degradation through the proteasome pathway.