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验证数据展示

  • IF Staining of C2C12 using 82941-1-RR (same clone as 82941-1-PBS)

    IF Staining of C2C12 using 82941-1-RR (same clone as 82941-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using FBXO32 antibody (82941-1-RR, Clone: 230243H7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82941-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 82941-1-RR (same clone as 82941-1-PBS)

    IHC staining of mouse skeletal muscle using 82941-1-RR (same clone as 82941-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82941-1-RR (FBXO32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82941-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FBXO32 antibody included
  • FC experiment of U-251 using 82941-1-RR (same clone as 82941-1-PBS)

    FC experiment of U-251 using 82941-1-RR (same clone as 82941-1-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human FBXO32 (82941-1-RR, Clone:230243H7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82941-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82941-1-RR

    Affinity and Kinetic Characterization of 82941-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82941-1-RR against Human FBXO32 were performed. The affinity constant is below 1 pM.

FBXO32 Recombinant antibody, PBS Only

FBXO32 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82941-1-PBS

产品说明书

CloneNo. 230243H7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

F-box only protein 32, F box protein 32, F box only protein 32, Atrogin-1, Atrogin 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy FBXO32 Ready-To-Use IHC Kit. FBXO32 primary antibody included.

产品信息

82941-1-PBS targets FBXO32 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 FBXO32 fusion protein Ag29394 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 F-box protein 32
别名 F-box only protein 32, F box protein 32, F box only protein 32, Atrogin-1, Atrogin 1
计算分子量 355 aa, 42 kDa
GenBank蛋白编号BC024030
基因名称 FBXO32
Gene ID (NCBI) 114907
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ969P5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FBXO32 (F box only protein 32), also known as Atrogin 1 or MAFbx, is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif F-box. This protein is an E3 ubiquitin ligase that is markedly up-regulated in muscle atrophy. FBXO32 is thus a potential drug target for the treatment of muscle atrophy. Some data support that FBXO32 may play an important role in tumorigenesis. Recent study reveal that FBXO32 targets the oncogenic protein c-Myc for ubiquitination and degradation through the proteasome pathway.