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  • Featured Product
  • KD/KO Validated

FBXO43 Polyclonal antibody

FBXO43 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 55176-1-AP

Print datasheet

Synonyms

FBXO 43, Fbx43, Fbp5B, F-box only protein 43, ERP1

验证数据展示

  • WB analysis of HEK-293 using 55176-1-AP

    WB analysis of HEK-293 using 55176-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55176-1-AP (FBXO43 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 55176-1-AP

    IF Staining of HEK-293 using 55176-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 55176-1-AP (FBXO43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55176-1-AP targets FBXO43 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name F-box protein 43
Synonyms FBXO 43, Fbx43, Fbp5B, F-box only protein 43, ERP1
Calculated Molecular Weight 78 kDa
Observed Molecular Weight ~70 kDa
GenBank Accession NumberNM_001029860
Gene Symbol FBXO43
Gene ID (NCBI) 286151
RRIDAB_2881282
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ4G163
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXO43, also named as EMI2 and FBP5B, is required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. It acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. FBXO43 recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. We got ~70 kDa band in our detection.

实验方案

Product Specific Protocols
WB protocol for FBXO43 antibody 55176-1-APDownload protocol
IF protocol for FBXO43 antibody 55176-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Sci Adv

SKP1 drives the prophase I to metaphase I transition during male meiosis.

Authors - Yongjuan Guan
View Article
humanWB,IHC,IF,IP

Cancer Lett

FBXO43 promotes cell cycle progression in cancer cells through stabilizing SKP2

Authors - Liyun Zheng
  • KD Validated
View Article
humanWB,IHC

Cancers (Basel)

F-Box Protein 43, Stabilized by N6-Methyladenosine Methylation, Enhances Hepatocellular Carcinoma Cell Growth and Invasion via Promoting p53 Degradation in a Ubiquitin Conjugating Enzyme E2 C-Dependent Manner

Authors - Huijun Zhou
  • KD Validated
View Article
humanWB,IHC

Front Oncol

FBXO43 increases CCND1 stability to promote hepatocellular carcinoma cell proliferation and migration

Authors - Chun-Ming Li
View Article