验证数据展示
经过测试的应用
Positive WB detected in | mouse liver tissue, rat liver tissue |
Positive IHC detected in | human liver cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
产品信息
10626-1-AP targets FBXO44 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | FBXO44 fusion protein Ag0977 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | F-box protein 44 |
别名 | F box only protein 44, F box protein 44, F box protein FBX30, F box/G domain protein 3, FBG3, FBX30, Fbx44, FBX6A, FBXO44, Fbxo6a |
计算分子量 | 26 kDa |
观测分子量 | 30-35 kDa |
GenBank蛋白编号 | BC007832 |
基因名称 | FBXO44 |
Gene ID (NCBI) | 93611 |
RRID | AB_2278465 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9H4M3 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FBXO44 is a member of the F-box protein family that shares a function as substrate recognition factors for the SKP1-CUL1-F-box (SCF)-type ubiquitin ligase. Several human FBXs (FBXO2, FBXO6, FBXO17, FBXO27, and FBXO44) share a conserved G domain (also known as FBA domain), which is responsible for recognizing N-glycan moieties by most FBXs in this group except FBXO44. (PMID: 25970626, PMID: 23086937)
实验方案
Product Specific Protocols | |
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WB protocol for FBXO44 antibody 10626-1-AP | Download protocol |
IHC protocol for FBXO44 antibody 10626-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |