验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11830-1-AP targets FBXO6 in IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 | FBXO6 fusion protein Ag2400 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | F-box protein 6 |
| 别名 | F box only protein 6, F box protein 6, F box/G domain protein 2, FBG2, FBS2, FBX6, Fbx6b, FBXO6 |
| 计算分子量 | 293 aa, 34 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC020880 |
| 基因名称 | FBXO6 |
| Gene ID (NCBI) | 26270 |
| RRID | AB_2104709 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NRD1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FBXO6(F-box only protein 6 ) is also named as FBG2, FBS2, FBX6 and belongs to the F-box protein family. It can regulate Chk1 ubiquitination and degradation in both normally cycling cells and during replication stress. This protein is mainly detected in the cytoplasm in both cultured cells and in tumor tissues. Its expression is a predictive biomarker of tumor responsiveness to the important anticancer drugs.(PMID:19716789). FBXO6 can inhibit the anaphase promoting complex (cyclosome APC) and prevent mitotic exit in cytostatic factor (CSF) arrested eggs as a mitotic regulator.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXO6 antibody 11830-1-AP | Download protocol |
| IHC protocol for FBXO6 antibody 11830-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
