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验证数据展示

  • WB analysis using 67697-1-Ig (same clone as 67697-1-PBS)

    WB analysis using 67697-1-Ig (same clone as 67697-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67697-1-Ig (FBXO6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67697-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 67697-1-Ig (same clone as 67697-1-PBS)

    WB analysis of Jurkat using 67697-1-Ig (same clone as 67697-1-PBS)

    WB result of FBXO6 antibody (67697-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBXO6 transfected Jurkat cells. This data was developed using the same antibody clone with 67697-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 67697-1-Ig (same clone as 67697-1-PBS)

    IF Staining of HEK-293 using 67697-1-Ig (same clone as 67697-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using FBXO6 antibody (67697-1-Ig, Clone: 3B7F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67697-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

FBXO6 Monoclonal antibody, PBS Only

FBXO6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67697-1-PBS

产品说明书

CloneNo. 3B7F9

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

3B7F9, F box only protein 6, F box protein 6, FBG2, F-box only protein 6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67697-1-PBS targets FBXO6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FBXO6 fusion protein Ag30512 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 F-box protein 6
别名 3B7F9, F box only protein 6, F box protein 6, FBG2, F-box only protein 6
计算分子量 293 aa, 34 kDa
观测分子量34 kDa
GenBank蛋白编号BC020880
基因名称 FBXO6
Gene ID (NCBI) 26270
RRIDAB_2882889
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NRD1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FBXO6(F-box only protein 6 ) is also named as FBG2, FBS2, FBX6 and belongs to the F-box protein family. FBXO6 is the substrate recognition component of a Skp1-Cullin1-F-box protein (SCF) ubiquitin E3 ligase complex, recognizing the chitobiose in unfolded N-glycoprotein to target glycoproteins for polyubiquitination and degradation. It can regulate Chk1 ubiquitination and degradation in both normally cycling cells and during replication stress. This protein is mainly detected in the cytoplasm in both cultured cells and in tumor tissues. Its expression is a predictive biomarker of tumor responsiveness to the important anticancer drugs. Positive Fbxo6 expression correlated with early TNM stage and favorable prognosis of NSCLC patients. (PMID:19716789, PMID: 27855403, PMID: 31140586).