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验证数据展示

  • WB analysis using 30458-1-AP

    WB analysis using 30458-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30458-1-AP (FBXO7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

FBXO7 Polyclonal antibody

FBXO7 Polyclonal Antibody for WB, ELISA
Cat No. 30458-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

DKFZp686B08113, F box only protein 7, F box protein 7, FBX, FBX07, FBX7, FBXO7, PARK15, PKPS

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, Raji cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30458-1-AP targets FBXO7 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen FBXO7 fusion protein Ag32796 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name F-box protein 7
Synonyms DKFZp686B08113, F box only protein 7, F box protein 7, FBX, FBX07, FBX7, FBXO7, PARK15, PKPS
Calculated Molecular Weight 59 kDa
Observed Molecular Weight59-75 kDa
GenBank Accession NumberBC008361
Gene Symbol FBXO7
Gene ID (NCBI) 25793
RRIDAB_3086324
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3I1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXO7, also named as FBX7, is a substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. FBXO7 recognizes BIRC2 and DLGAP5. It function in phosphorylation-dependent ubiquitination.

实验方案

Product Specific Protocols
WB protocol for FBXO7 antibody 30458-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols