验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, Raji cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30458-1-AP targets FBXO7 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | FBXO7 fusion protein Ag32796 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | F-box protein 7 |
| 别名 | DKFZp686B08113, F box only protein 7, F box protein 7, FBX, FBX07, FBX7, FBXO7, PARK15, PKPS |
| 计算分子量 | 59 kDa |
| 观测分子量 | 59-75 kDa |
| GenBank蛋白编号 | BC008361 |
| 基因名称 | FBXO7 |
| Gene ID (NCBI) | 25793 |
| RRID | AB_3086324 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y3I1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FBXO7, also named as FBX7, is a substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. FBXO7 recognizes BIRC2 and DLGAP5. It function in phosphorylation-dependent ubiquitination.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXO7 antibody 30458-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |