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FBXW12 Polyclonal antibody

FBXW12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21687-1-AP

Print datasheet

Synonyms

F box only protein 35, Fbw12, FBXO12, FBXO35, FBXW12

验证数据展示

  • WB analysis of K-562 using 21687-1-AP

    WB analysis of K-562 using 21687-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 21687-1-AP (FBXW12 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 21687-1-AP

    IHC staining of human breast cancer using 21687-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21687-1-AP (FBXW12 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 21687-1-AP

    IHC staining of human breast cancer using 21687-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21687-1-AP (FBXW12 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21687-1-AP is FBXW12 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen FBXW12 fusion protein Ag16266 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name F-box and WD repeat domain containing 12
Synonyms F box only protein 35, Fbw12, FBXO12, FBXO35, FBXW12
Calculated Molecular Weight 464 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC101304
Gene Symbol FBXW12
Gene ID (NCBI) 285231
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6X9E4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.