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FBXW7 Polyclonal antibody
FBXW7 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse and More (3)
Applications
WB, IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, MCF-7 cells, mouse brain tissue |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy FBXW7 Ready-To-Use IHC Kit. FBXW7 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28424-1-AP targets FBXW7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | Human, Mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | FBXW7 fusion protein Ag28471 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | F-box and WD repeat domain containing 7 |
| Synonyms | AGO, Archipelago homolog, CDC4, DKFZp686F23254, F box protein FBX30, FBW6, FBW7, FBX30, FBXO30, FBXW6, FBXW7, FLJ16457, hAgo, hCdc4, SEL 10, SEL10 |
| Calculated Molecular Weight | 66 kDa |
| Observed Molecular Weight | 100-110 kDa, 66-75 kDa |
| GenBank Accession Number | NM_001349798 |
| Gene Symbol | FBXW7 |
| Gene ID (NCBI) | 55294 |
| RRID | AB_2881138 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q969H0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FBXW7, also named as FBW7, FBX30, SEL10 and hAgo, is a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It probably recognizes and binds to phosphorylated target proteins. FBXW7 is involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. FBXW7 is a general tumor suppressor in human cancer (PMID: 17909001). FBXW7 has 3 isoforms (α/β/γ) with the calculated molecular mass of 80 kDa, 70 kDa and 66 kDa, and apparent molecular mass of 100-110 kDa and 66-75 kDa (PMID: 31346036/PMID: 22864569). K48 autoubiquitination is consistent with maintenance of ubiquitinated FBW7 (Ub-FBW7, 200 kDa)(PMID: 32353058).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXW7 antibody 28424-1-AP | Download protocol |
| IHC protocol for FBXW7 antibody 28424-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer Loss of FBXW7-mediated degradation of BRAF elicits resistance to BET inhibitors in adult T cell leukemia cells. | ||
J Exp Med Somatic gain-of-function mutations in BUD13 promote oncogenesis by disrupting Fbw7 function | ||
Theranostics Histone demethylase JMJD3 protects against renal fibrosis by suppressing TGFβ and Notch signaling and preserving PTEN expression. | ||
EMBO Rep The 18S rRNA m6 A methyltransferase METTL5 promotes mouse embryonic stem cell differentiation. | ||
Free Radic Biol Med NOX1 triggers ferroptosis and ferritinophagy, contributes to Parkinson's disease | ||
Int Immunopharmacol MiR-223-3p regulates the eosinophil degranulation and enhances the inflammation in allergic rhinitis by targeting FBXW7 |
