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验证数据展示

  • WB analysis using 13566-1-AP

    WB analysis using 13566-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13566-1-AP (FCER1G antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 13566-1-AP

    IHC staining of human ovary cancer using 13566-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13566-1-AP (FCER1G antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of THP-1 using 13566-1-AP

    IF Staining of THP-1 using 13566-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using FCER1G antibody (13566-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

FCER1G Polyclonal antibody

FCER1G Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13566-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

FcRgamma, FCRG, FceRI gamma, Fc-epsilon RI-gamma, Fc receptor gamma-chain

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRAW 264.7 cells, THP-1 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13566-1-AP targets FCER1G in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 FCER1G fusion protein Ag4442 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide
别名 FcRgamma, FCRG, FceRI gamma, Fc-epsilon RI-gamma, Fc receptor gamma-chain
计算分子量 87 aa, 10 kDa
观测分子量10 kDa
GenBank蛋白编号BC033872
基因名称 FcRγ
Gene ID (NCBI) 2207
RRIDAB_3669165
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP30273
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FCER1G (Fc receptor gamma-chain, FcRgamma), also known as Fc-epsilon RI-gamma, or IgE Fc receptor subunit gamma (FceRI gamma), is a component of the high-affinity immunoglobulin E (IgE) receptor (Fc epsilon RI) which is a tetrameric complex of one alpha subunit, one beta subunit, and two disulfide-linked gamma subunits (PMID: 2146219; 2531187). Fc epsilon RI is present exclusively on mast cells and basophils, mediating allergic inflammatory signaling (PMID: 17438574). FCER1G is widely expressed and contains an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors (PMID: 19098920).

实验方案

Product Specific Protocols
WB protocol for FCER1G antibody 13566-1-APDownload protocol
IHC protocol for FCER1G antibody 13566-1-APDownload protocol
IF protocol for FCER1G antibody 13566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols