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CD64 Polyclonal antibody

CD64 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27563-1-AP

Print datasheet

Synonyms

CD64, CD64A, Fc gamma RI, Fc gamma RIA, FCG1, FcgammaRIa, FCGR1, FCGR1A, FCRI, IGFR1, IgG Fc receptor I

验证数据展示

  • WB analysis using 27563-1-AP

    WB analysis using 27563-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 27563-1-AP (CD64 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 27563-1-AP

    IF Staining of THP-1 using 27563-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD64 antibody (27563-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inTHP-1 cells, U-937 cells, HL-60 cells
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27563-1-AP targets CD64 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen CD64 fusion protein Ag26727 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fc fragment of IgG, high affinity Ia, receptor (CD64)
Synonyms CD64, CD64A, Fc gamma RI, Fc gamma RIA, FCG1, FcgammaRIa, FCGR1, FCGR1A, FCRI, IGFR1, IgG Fc receptor I
Calculated Molecular Weight 374 aa, 43 kDa
Observed Molecular Weight 65-70 kDa, 43-45 kDa
GenBank Accession NumberBC032634
Gene Symbol FCGR1A
Gene ID (NCBI) 2209
ENSEMBL Gene IDENSG00000150337
RRIDAB_2880909
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12314
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses. The calculated molecular weight of CD64 is 43 kDa, while the glycosylated CD64 has a higher apparent molecular weight.

实验方案

Product Specific Protocols
WB protocol for CD64 antibody 27563-1-APDownload protocol
IF protocol for CD64 antibody 27563-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

Cancer Cell-Derived Exosomes Promote HCC Tumorigenesis Through Hedgehog Pathway.

Authors - Li Li
View Article