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验证数据展示

  • WB analysis using 27138-1-AP

    WB analysis using 27138-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27138-1-AP (FCGR2A / CD32a antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 27138-1-AP

    IHC staining of human tonsillitis using 27138-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27138-1-AP (FCGR2A / CD32a antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FCGR2A/CD32A antibody included
  • IHC staining of human tonsillitis using 27138-1-AP

    IHC staining of human tonsillitis using 27138-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27138-1-AP (FCGR2A / CD32a antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FCGR2A/CD32A antibody included

FCGR2A / CD32a Polyclonal antibody

FCGR2A / CD32a Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27138-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CD32, Fc-gamma RII-a, FCG2, Fc gamma RIIa, Fc gamma RII a

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inU-937 cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FCGR2A/CD32A Ready-To-Use IHC Kit. FCGR2A/CD32A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27138-1-AP targets FCGR2A / CD32a in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen FCGR2A / CD32a fusion protein Ag25878 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fc fragment of IgG, low affinity IIa, receptor (CD32)
Synonyms CD32, Fc-gamma RII-a, FCG2, Fc gamma RIIa, Fc gamma RII a
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC020823
Gene Symbol CD32a
Gene ID (NCBI) 2212
RRIDAB_2880770
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

实验方案

Product Specific Protocols
WB protocol for FCGR2A / CD32a antibody 27138-1-APDownload protocol
IHC protocol for FCGR2A / CD32a antibody 27138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols