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FCGR2A / CD32a Monoclonal antibody, PBS Only

FCGR2A / CD32a Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D3F1

Cat no : 66529-1-PBS

Print datasheet

Synonyms

CD32, CD32A, CDw32, Fc gamma RII a, Fc gamma RIIa, FCG2, FcGR, FCGR2, FCGR2A, FCGR2A1, FcRII a, IGFR2, IgG Fc receptor II a

验证数据展示

  • WB analysis using 66529-1-Ig (same clone as 66529-1-PBS)

    WB analysis using 66529-1-Ig (same clone as 66529-1-PBS)

    Untreated and PNGase F-treated lysates of THP-1 cells and Mo7e cells were subjected to SDS PAGE followed by western blot with 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66529-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66529-1-Ig (same clone as 66529-1-PBS)

    IHC staining of human liver cancer using 66529-1-Ig (same clone as 66529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66529-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FCGR2A/CD32A antibody included
  • IHC staining of human liver cancer using 66529-1-Ig (same clone as 66529-1-PBS)

    IHC staining of human liver cancer using 66529-1-Ig (same clone as 66529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66529-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FCGR2A/CD32A antibody included
  • IF Staining of THP-1 using 66529-1-Ig (same clone as 66529-1-PBS)

    IF Staining of THP-1 using 66529-1-Ig (same clone as 66529-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using FCGR2A / CD32a antibody (66529-1-Ig, Clone: 1D3F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66529-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy FCGR2A/CD32A Ready-To-Use IHC Kit. FCGR2A/CD32A primary antibody included.

产品信息

66529-1-PBS targets FCGR2A / CD32a in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen FCGR2A / CD32a fusion protein Ag25878 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name Fc fragment of IgG, low affinity IIa, receptor (CD32)
Synonyms CD32, CD32A, CDw32, Fc gamma RII a, Fc gamma RIIa, FCG2, FcGR, FCGR2, FCGR2A, FCGR2A1, FcRII a, IGFR2, IgG Fc receptor II a
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC020823
Gene Symbol CD32a
Gene ID (NCBI) 2212
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12318
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.