验证数据展示
经过测试的应用
Positive WB detected in | HL-60 cells, Jurkat cells |
Positive IF-P detected in | mouse spleen tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30356-1-AP targets FCGR3A/CD16a in WB, IF-P, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IF-P, ELISA Application Description |
经测试反应性 | human |
免疫原 | FCGR3A/CD16a fusion protein Ag1281 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | Fc fragment of IgG, low affinity IIIa, receptor (CD16a) |
别名 | CD16, Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen |
计算分子量 | 29 kDa |
观测分子量 | 65-70 kDa |
GenBank蛋白编号 | BC036723 |
基因名称 | CD16a |
Gene ID (NCBI) | 2214 |
ENSEMBL Gene ID | ENSG00000203747 |
RRID | AB_3086300 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P08637 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins(PMID: 30361439). FCGR3A has a calculated molecular weight of 29 kDa, with an apparent molecular weight of 50-80 kDa due to extensive glycosylation (PMID: 30655272; PMID: 27914599). The antibody is specific for FCGR3A/CD16a.
实验方案
Product Specific Protocols | |
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WB protocol for FCGR3A/CD16a antibody 30356-1-AP | Download protocol |
IF protocol for FCGR3A/CD16a antibody 30356-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |