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验证数据展示

  • WB analysis using 30201-1-AP

    WB analysis using 30201-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30201-1-AP (FCGR3B/CD16b antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 30201-1-AP

    IF Staining of A549 using 30201-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using FCGR3B/CD16b antibody (30201-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

FCGR3B/CD16b Polyclonal antibody

FCGR3B/CD16b Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30201-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, rat

应用

WB, IF/ICC, ELISA

别名

CD16, CD16b, Fc gamma RIII, Fc gamma RIII beta, Fc gamma RIIIb, FCG3, FCGR3, FCGR3B, FcR 10, FcRIII, FcRIIIb, IGFR3, IgG Fc receptor III 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, U-937 cells, rat spleen tissue
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30201-1-AP targets FCGR3B/CD16b in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, rat
免疫原 fusion protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
别名 CD16, CD16b, Fc gamma RIII, Fc gamma RIII beta, Fc gamma RIIIb, FCG3, FCGR3, FCGR3B, FcR 10, FcRIII, FcRIIIb, IGFR3, IgG Fc receptor III 1
计算分子量26 kDa
观测分子量55 kDa
GenBank蛋白编号NM_000570
基因名称 FCGR3B
Gene ID (NCBI) 2215
RRIDAB_3086262
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75015
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins(PMID: 30361439). FCGR3B/CD16b can be detected as 26 kDa (PMID: 34108956; 36096917) and 50-75 kDa (PMID: 30655272; 18208407). The antibody is specific for FCGR3B/CD16b.

实验方案

Product Specific Protocols
WB protocol for FCGR3B/CD16b antibody 30201-1-APDownload protocol
IF protocol for FCGR3B/CD16b antibody 30201-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols