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FCGR3B/CD16b Polyclonal antibody

FCGR3B/CD16b Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30201-1-AP

Print datasheet

Synonyms

CD16, CD16b, Fc gamma RIII, Fc gamma RIII beta, Fc gamma RIIIb, FCG3, FCGR3, FCGR3B, FcR 10, FcRIII, FcRIIIb, IGFR3, IgG Fc receptor III 1

验证数据展示

  • WB analysis using 30201-1-AP

    WB analysis using 30201-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30201-1-AP (FCGR3B/CD16b antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 30201-1-AP

    IF Staining of A549 using 30201-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using FCGR3B/CD16b antibody (30201-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inRaji cells, U-937 cells, rat spleen tissue
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30201-1-AP targets FCGR3B/CD16b in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, rat
Immunogen fusion protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
Synonyms CD16, CD16b, Fc gamma RIII, Fc gamma RIII beta, Fc gamma RIIIb, FCG3, FCGR3, FCGR3B, FcR 10, FcRIII, FcRIIIb, IGFR3, IgG Fc receptor III 1
Calculated Molecular Weight26 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberNM_000570
Gene Symbol FCGR3B
Gene ID (NCBI) 2215
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75015
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins(PMID: 30361439). FCGR3B/CD16b can be detected as 26 kDa (PMID: 34108956; 36096917) and 50-75 kDa (PMID: 30655272; 18208407). The antibody is specific for FCGR3B/CD16b.

实验方案

Product Specific Protocols
WB protocol for FCGR3B/CD16b antibody 30201-1-APDownload protocol
IF protocol for FCGR3B/CD16b antibody 30201-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols