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验证数据展示

  • WB analysis of Raji using 26949-1-AP

    WB analysis of Raji using 26949-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 26949-1-AP (FCRLA Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 26949-1-AP

    IHC staining of human tonsillitis using 26949-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26949-1-AP (FCRLA antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 26949-1-AP

    IHC staining of human tonsillitis using 26949-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26949-1-AP (FCRLA antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FCRLA Polyclonal antibody

FCRLA Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26949-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

FCRL, Fc receptor-related protein X, Fc receptor-like protein, Fc receptor-like and mucin-like protein 1, Fc receptor-like A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, Daudi cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26949-1-AP targets FCRLA in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 FCRLA fusion protein Ag25331 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Fc receptor-like A
别名 FCRL, Fc receptor-related protein X, Fc receptor-like protein, Fc receptor-like and mucin-like protein 1, Fc receptor-like A
计算分子量 39 kDa
观测分子量 39-43 kDa
GenBank蛋白编号BC006521
基因名称 FCRLA
Gene ID (NCBI) 84824
RRIDAB_2880697
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7L513
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FCRLA antibody 26949-1-APDownload protocol
IHC protocol for FCRLA antibody 26949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols