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Host
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Species Reactivity
0
Species Reactivity
0
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0
Conjugate
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验证数据展示

  • WB analysis using 83020-1-RR (same clone as 83020-1-PBS)

    WB analysis using 83020-1-RR (same clone as 83020-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83020-1-RR (FDFT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83020-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83020-1-RR (same clone as 83020-1-PBS)

    IHC staining of human ovary cancer using 83020-1-RR (same clone as 83020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83020-1-RR (FDFT1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83020-1-PBS in a different storage buffer formulation.

  • IF Staining of L02 using 83020-1-RR (same clone as 83020-1-PBS)

    IF Staining of L02 using 83020-1-RR (same clone as 83020-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using FDFT1 antibody (83020-1-RR, Clone: 230362C10 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83020-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83020-1-RR (same clone as 83020-1-PBS)

    FC experiment of U2OS using 83020-1-RR (same clone as 83020-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human FDFT1 (83020-1-RR, Clone:230362C10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83020-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83020-1-RR

    Affinity and Kinetic Characterization of 83020-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83020-1-RR against Human FDFT1 were performed. The affinity constant is 0.126 nM.

FDFT1 Recombinant antibody, PBS Only

FDFT1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83020-1-PBS

产品说明书

CloneNo. 230362C10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

Farnesyl-diphosphate farnesyltransferase 1, Farnesyl-diphosphate farnesyltransferase, ERG9, EC:2.5.1.21, DGPT

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83020-1-PBS targets FDFT1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 FDFT1 fusion protein Ag3743 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 farnesyl-diphosphate farnesyltransferase 1
别名 Farnesyl-diphosphate farnesyltransferase 1, Farnesyl-diphosphate farnesyltransferase, ERG9, EC:2.5.1.21, DGPT
计算分子量 417 aa, 48 kDa
观测分子量48 kDa
GenBank蛋白编号BC029641
基因名称 FDFT1
Gene ID (NCBI) 2222
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP37268
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SQS (Squalene synthase), also known as Farnesyl-diphosphate farnesyltransferase 1 (FDFT1) is a gene that encodes the membrane-associated enzyme squalene synthase, which is the first specific enzyme in cholesterol biosynthesis. FDFT1 is highly expressed in liver, lung, prostate, breast, ovary, bladder, cervix, thyroid, and esophageal cancers, while in colorectal, colon, testicular, uterine, pancreas, and kidney tumors, its expression is downregulated (PMID: 35093030). FDFT1 is a key downstream target of the fasting response and may be involved in CRC cell glucose metabolism (PMID: 32313017).