验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, pig adrenal gland tissue, human adrenal gland tissue, rat testis tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
15584-1-AP targets FDXR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human |
| 免疫原 | FDXR fusion protein Ag7145 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ferredoxin reductase |
| 别名 | Adrenodoxin reductase, ADXR, Aerredoxin Reductase, AR, FDXR, Ferredoxin NADP(+) reductase |
| 计算分子量 | 54 kDa |
| 观测分子量 | 48-58 kDa |
| GenBank蛋白编号 | BC063493 |
| 基因名称 | FDXR |
| Gene ID (NCBI) | 2232 |
| RRID | AB_2102602 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P22570 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ferredoxin reductase (FDXR, also known as adrenodoxin reductase) is a mitochondrial membrane-associated flavoprotein. One of its functions is to transfer electrons from NADPH to the two human ferredoxin proteins, FDX1 and FDX2. FDXR is expressed in all tissues with the highest expression in tissues such as the adrenal cortex, that specialize in steroid hormone synthesis. Functionally, FDXR is suggested to be involved in various Functionally, FDXR is suggested to be involved in various. Notably, recent studies have shown that FDXR mutations are associated with mitochondrial disorders, probably due to its role in iron-sulfur cluster protein biosynthesis (PMID: 32304229). FDXR has 7 isoforms with the molecular weight of 48-58 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FDXR antibody 15584-1-AP | Download protocol |
| IHC protocol for FDXR antibody 15584-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem The neurosteroid pregnenolone is synthesized by a mitochondrial P450 enzyme other than CYP11A1 in human glial cells. | ||
Front Oncol FDXR drives primary and endocrine-resistant tumor cell growth in ER+ breast cancer via CPT1A-mediated fatty acid oxidation
|
