FECH Polyclonal antibody

FECH Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14466-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA

EPP, FCE, FECH, Ferrochelatase, mitochondrial, Heme synthase, Protoheme ferro lyase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse heart tissue, human liver tissue, HepG2 cells, human heart tissue, K-562 cells, mouse kidney tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14466-1-AP targets FECH in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 FECH fusion protein Ag5814 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ferrochelatase (protoporphyria)
别名 EPP, FCE, FECH, Ferrochelatase, mitochondrial, Heme synthase, Protoheme ferro lyase
计算分子量 48 kDa
观测分子量 40-48 kDa
GenBank蛋白编号BC039841
基因名称 FECH
Gene ID (NCBI) 2235
RRIDAB_2231579
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP22830
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FECH(ferrochelatase, mitochondrial ) is also named as heme synthase, protoheme ferro-lyase and belongs to the ferrochelatase family. It is a homodimeric (86 kDa) mitochondrial membrane-associated enzyme that catalyzes the insertion of ferrous iron into protoporphyrin to form heme(PMID:11175906). It has 2 isoforms produced by alternative splicing and the full length protein has a transit peptide with 54 amino acids.

实验方案

Product Specific Protocols
WB protocol for FECH antibody 14466-1-APDownload protocol
IHC protocol for FECH antibody 14466-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.

Authors - Ying Wang
humanWB

Cancer Discov

NCOA4-Mediated Ferritinophagy Is a Pancreatic Cancer Dependency via Maintenance of Iron Bioavailability for Iron-Sulfur Cluster Proteins

Authors - Naiara Santana-Codina
humanWB

Cell Metab

A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III.

Authors - Nunziata Maio
humanWB

Mol Cell

Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.

Authors - Ross A Weber
humanWB

J Am Coll Cardiol

Heme levels are increased in human failing hearts.

Authors - Khechaduri Arineh A
mouseWB

Nat Commun

PMID: 32019928

Authors - Erika M Palmieri
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