FEM1B Polyclonal antibody
FEM1B Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse testis tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
11030-1-AP targets FEM1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | FEM1B fusion protein Ag1497 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | fem-1 homolog b (C. elegans) |
| Synonyms | FEM1-beta, FEM1 beta, fem 1 homolog b (C. elegans), F1A-alpha, F1AA |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 61 kDa |
| GenBank Accession Number | BC010122 |
| Gene Symbol | FEM1B |
| Gene ID (NCBI) | 10116 |
| RRID | AB_2102637 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UK73 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FEM1B, also named F1AA, and KIAA0396 is a 627 amino acid protein belonging to the fem-1 family. FEM1B is highly expressed in the testis and weakly expressed in other tissues. FEM1B localizes both in the nucleus and cytoplasm. In the nucleus, the protein level increased slightly after camptothecin (CPT) treatment. FEM1B is a component of an E3 ubiquitin-protein ligase complex, which may act as a substrate recognition subunit. FEM1B is involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. FEM1B is also involved in glucose homeostasis in pancreatic islets. FEM1B functions as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FEM1B antibody 11030-1-AP | Download protocol |
| IHC protocol for FEM1B antibody 11030-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |