验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse testis tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
11030-1-AP targets FEM1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | FEM1B fusion protein Ag1497 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fem-1 homolog b (C. elegans) |
| 别名 | FEM1-beta, FEM1 beta, fem 1 homolog b (C. elegans), F1A-alpha, F1AA |
| 计算分子量 | 70 kDa |
| 观测分子量 | 61 kDa |
| GenBank蛋白编号 | BC010122 |
| 基因名称 | FEM1B |
| Gene ID (NCBI) | 10116 |
| RRID | AB_2102637 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UK73 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FEM1B, also named F1AA, and KIAA0396 is a 627 amino acid protein belonging to the fem-1 family. FEM1B is highly expressed in the testis and weakly expressed in other tissues. FEM1B localizes both in the nucleus and cytoplasm. In the nucleus, the protein level increased slightly after camptothecin (CPT) treatment. FEM1B is a component of an E3 ubiquitin-protein ligase complex, which may act as a substrate recognition subunit. FEM1B is involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. FEM1B is also involved in glucose homeostasis in pancreatic islets. FEM1B functions as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FEM1B antibody 11030-1-AP | Download protocol |
| IHC protocol for FEM1B antibody 11030-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |